Z2
OverviewIn project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.
SEEK ID: https://funginet.hki-jena.de/projects/35
Public web page: http://www.funginet.de/project-z2.html
Organisms: Aspergillus fumigatus, Candida albicans
INF PALs: No PALs for this Project
Project created: 4th Mar 2016
Related items
Institutions: BioControl Jena GmbH
: inactive since 2020-06-01
Projects: FungiNet total, INF, A1, A2, A3, A4 (E), A5, A6, B1, B3 (E), B4, B5, C1, C2, C3, C4 (E), C5, C6 (E), Z1, Z2, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, B2
Institutions: Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)
https://orcid.org/0000-0001-6106-724X
I am responisble for the spatial proteomics part in Z2. We provide a proteomic platform to study spatial changes of the proteome during host-fungal pathogen interaction using MALDI-MS Imaging and Lasermicrodissection. As type of biological sample we can work with cryo and formalin fixed tissue, as well as cell cultures and lysed sample solutions. Please get into contact with me if you are interested in MALDI-MS Imaging for your research project.
Head of the subgroup Stress and Immunoproteomics of the Department of Molecular and Applied Microbiology Department at the HKI
The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...
Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7
Web page: http://www.funginet.de/home.html
P Hortschansky, M Eisendle, Q Al-Abdallah, A D Schmidt, S Bergmann, M Thön, O Kniemeyer, B Abt, B Seeber, E R Werner, M Kato, A A Brakhage and H Haas. Interaction of HapX with the CCAAT-binding complex-a novel mechanism of gene regulation by iron. EMBO J. 2007 Jul 11;26(13):3157-68. Epub 2007 Jun 14
Wartenberg D, Vödisch M, Kniemeyer O, Albrecht-Eckardt D, Scherlach K, Winkler R, Weide M, Brakhage AA. Proteome analysis of the farnesol-induced stress response in Aspergillus nidulans-The role of a putative dehydrin. J Proteomics. 2012 May 23.
Submitter: Daniela Albrecht-Eckardt
Studies: Control, Farnesol Treatment, Internal Standard
Assays: Control gels, Farnesol gels, Standard gels
D Albrecht, R Guthke, A A Brakhage, O Kniemeyer. Integrative analysis of the heat shock response in Aspergillus fumigatus. BMC Genomics. 2010 Jan 15;11:32.
Find corresponding transcriptomic data at ArrayExpress: E-MEXP-332
Submitter: Daniela Albrecht-Eckardt
Studies: pooled standard, t=0, t=120, t=180, t=30, t=60
Assays: pooled standard gels, t0 gels, t120 gels, t180 gels, t30 gels, t60 gels
Dirk Wartenberg, Katrin Lapp, Ilse D. Jacobsen, Hans-Martin Dahse, Olaf Kniemeyer, Thorsten Heinekamp, Axel A. Brakhage Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knoell-Institute (HKI), Jena, Germany. Secretome analysis of Aspergillus fumigatus reveals Asp-hemolysin as a major secreted protein. Int J Med Microbiol. 2011, Nov;301(7):602-11
Teutschbein J, Simon S, Lother J, Springer J, Hortschansky P, Morton CO, Löffler J, Einsele H, Conneally E, Rogers TR, Guthke R, Brakhage AA, Kniemeyer O. Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis. J Proteome Res 2016 Mar 24
.
Aspergillus nidulans R21 was grown over night in AMM at 37°C and 200 rpm. The culture was treated with 50 µM farnesol or the same volume of ethanol without farnesol as control. The culture was harvested after 3 hours of further incubation.
Submitter: Daniela Albrecht-Eckardt
Assay type: Experimental Assay Type
Technology type: 2D Gel Electrophoresis
Investigation: Proteome Map of Aspergillus fumigatus
Study: Conidia
Submitter: Daniela Albrecht-Eckardt
Assay type: Experimental Assay Type
Technology type: 2D Gel Electrophoresis
Investigation: Proteome Map of Aspergillus fumigatus
Study: Conidia
Submitter: Daniela Albrecht-Eckardt
Assay type: Experimental Assay Type
Technology type: 2D Gel Electrophoresis
Investigation: Proteome Map of Aspergillus fumigatus
Study: Conidia
Submitter: Daniela Albrecht-Eckardt
Assay type: Experimental Assay Type
Technology type: 2D Gel Electrophoresis
Investigation: Proteome Map of Aspergillus fumigatus
Study: Conidia
Submitter: Daniela Albrecht-Eckardt
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteome Map of Aspergillus fumigatus
Study: Mitochondrion
Creator: Daniela Albrecht-Eckardt
Submitter: Daniela Albrecht-Eckardt
DownloadCreator: Daniela Albrecht-Eckardt
Submitter: Daniela Albrecht-Eckardt
Creator: Daniela Albrecht-Eckardt
Submitter: Daniela Albrecht-Eckardt
Creator: Daniela Albrecht-Eckardt
Submitter: Daniela Albrecht-Eckardt
Creator: Daniela Albrecht-Eckardt
Submitter: Daniela Albrecht-Eckardt
Creator: Franziska Hoffmann
Submitter: Franziska Hoffmann
Creator: Franziska Hoffmann
Submitter: Franziska Hoffmann
Creators: Franziska Hoffmann, Daniela Pelzel
Submitter: Franziska Hoffmann
Creators: Franziska Hoffmann, Daniela Pelzel
Submitter: Franziska Hoffmann
Creator: Franziska Hoffmann
Submitter: Franziska Hoffmann
Abstract (Expand)
Authors: A. A. Kelani, A. Bruch, F. Rivieccio, C. Visser, T. Kruger, D. Weaver, X. Pan, S. Schauble, G. Panagiotou, O. Kniemeyer, M. J. Bromley, P. Bowyer, A. E. Barber, A. A. Brakhage, M. G. Blango
Date Published: 19th Jun 2023
Publication Type: Journal
Abstract (Expand)
Authors: B. Tappe, C. D. Lauruschkat, L. Strobel, J. Pantaleon Garcia, O. Kurzai, S. Rebhan, S. Kraus, E. Pfeuffer-Jovic, L. Bussemer, L. Possler, M. Held, K. Hunniger, O. Kniemeyer, S. Schauble, A. A. Brakhage, G. Panagiotou, P. L. White, H. Einsele, J. Loffler, S. Wurster
Date Published: 2nd Sep 2022
Publication Type: Journal
Abstract (Expand)
Authors: P. Brandt, F. Gerwien, L. Wagner, T. Kruger, B. Ramirez-Zavala, M. H. Mirhakkak, S. Schauble, O. Kniemeyer, G. Panagiotou, A. A. Brakhage, J. Morschhauser, S. Vylkova
Date Published: 23rd May 2022
Publication Type: Journal
Abstract (Expand)
Authors: L. J. de Assis, L. P. Silva, O. Bayram, P. Dowling, O. Kniemeyer, T. Kruger, A. A. Brakhage, Y. Chen, L. Dong, K. Tan, K. H. Wong, L. N. A. Ries, G. H. Goldman
Date Published: 5th Jan 2021
Publication Type: Not specified
Abstract (Expand)
Authors: M. I. A. Hassan, J. M. Kruse, T. Kruger, H. M. Dahse, Z. Cseresnyes, M. G. Blango, H. Slevogt, F. Horhold, V. Ast, R. Konig, M. T. Figge, O. Kniemeyer, A. A. Brakhage, K. Voigt
Date Published: 26th Jun 2020
Publication Type: Not specified
