MALDI-Imaging workflow for cryo tissue sections Version 1
SEEK ID: https://funginet.hki-jena.de/sops/17?version=1
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Z2_MSI_Cryo-Trypsin-Workflow.pdf
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Created: 17th Feb 2021 at 09:22
Last updated: 17th Feb 2021 at 11:18
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Version 1 (earliest) Created 17th Feb 2021 at 09:22 by Franziska Hoffmann
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https://orcid.org/0000-0002-6872-924XI am responisble for the spatial proteomics part in Z2. We provide a proteomic platform to study spatial changes of the proteome during host-fungal pathogen interaction using MALDI-MS Imaging and Lasermicrodissection. As type of biological sample we can work with cryo and formalin fixed tissue, as well as cell cultures and lysed sample solutions. Please get into contact with me if you are interested in MALDI-MS Imaging for your research project.
The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...
Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7
Web page: http://www.funginet.de/home.html
In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.
