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Franziska Hoffmann

About Franziska Hoffmann:

I am responisble for the spatial proteomics part in Z2. We provide a proteomic platform to study spatial changes of the proteome during host-fungal pathogen interaction using MALDI-MS Imaging and Lasermicrodissection. As type of biological sample we can work with cryo and formalin fixed tissue, as well as cell cultures and lysed sample solutions.
Please get into contact with me if you are interested in MALDI-MS Imaging for your research project.

SEEK ID: https://funginet.hki-jena.de/people/177

Location:  Germany

Expertise: MALDI-MS Imaging, Proteomics

Tools: Cell and tissue culture, Cell biology, Image analysis

ORCID: https://orcid.org/0000-0002-6872-924X

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Project positions

Z2 (University Hospital Jena)

Related items

Z2

In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-z2.html

University Hospital Jena

Country:  Germany

City: 07747 Jena

Web page: Not specified

Spatial Proteomics
Z2

This service and research topic deals with the spatially resolved field of proteomics which allows targetet and untargeted host-pathogen interaction studies.
Please get into contact with Franziska Hoffmann and Ferdinand von Eggeling if you are interested in spatial proteomics.

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Identification of fungal species in patient tissue using MALDI-Imaging and Deep Learning approaches
Z2
No description specified

Person responsible: Franziska Hoffmann

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Visualization and detection of CwhA in Aspergillus infection
Z2
No description specified

Person responsible: Franziska Hoffmann

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Visualization of HsbA in murine and human Lichtheimia infection
Z2
No description specified

Person responsible: Franziska Hoffmann

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PAS staining of tissue sections
Z2
No description specified

Creator: Franziska Hoffmann

Contributor: Franziska Hoffmann

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H&E staining of tissue sections
Z2
No description specified

Creator: Franziska Hoffmann

Contributor: Franziska Hoffmann

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MALDI-Imaging workflow for cryo tissue sections
Z2
No description specified

Creators: Franziska Hoffmann, Daniela Pelzel

Contributor: Franziska Hoffmann

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MALDI-Imaging workflow for FFPE tissue sections
Z2
No description specified

Creators: Franziska Hoffmann, Daniela Pelzel

Contributor: Franziska Hoffmann

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Formalin Fixation and Paraffin Embedding of tissue samples
Z2
No description specified

Creator: Franziska Hoffmann

Contributor: Franziska Hoffmann

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Microdissection-An Essential Prerequisite for Spatial Cancer Omics.
Z2

Abstract (Expand)

The problem with cancer tissue is that its intratumoral heterogeneity and its complexity is extremely high as cells possess, depending on their location and function, different mutations, different … mRNA expression and the highest intricacy in the protein pattern. Prior to genomic and proteomic analyses, it is therefore indispensable to identify the exact part of the tissue or even the exact cell. Laser-based microdissection is a tried and tested technique able to produce pure and well-defined cell material for further analysis with proteomic and genomic techniques. It sheds light on the heterogeneity of cancer or other complex diseases and enables the identification of biomarkers. This review aims to raise awareness for the reconsideration of laser-based microdissection and seeks to present current state-of-the-art combinations with omic techniques.

Authors: Ferdinand Von Eggeling, Franziska Hoffmann

Date Published: 25th Jun 2020

Journal: Proteomics

Identification of Proteomic Markers in Head and Neck Cancer Using MALDI-MS Imaging, LC-MS/MS, and Immunohistochemistry.
Z2

Abstract (Expand)

PURPOSE: The heterogeneity of squamous cell carcinoma tissue greatly complicates diagnosis and individualized therapy. Therefore, characterizing the heterogeneity of tissue spatially and identifying … appropriate biomarkers is crucial. MALDI-MS imaging (MSI) is capable of analyzing spatially resolved tissue biopsies on a molecular level. EXPERIMENTAL DESIGN: MALDI-MSI is used on snap frozen and formalin-fixed and paraffin-embedded (FFPE) tissue samples from patients with head and neck cancer (HNC) to analyze m/z values localized in tumor and nontumor regions. Peptide identification is performed using LC-MS/MS and immunohistochemistry (IHC). RESULTS: In both FFPE and frozen tissue specimens, eight characteristic masses of the tumor's epithelial region are found. Using LC-MS/MS, the peaks are identified as vimentin, keratin type II, nucleolin, heat shock protein 90, prelamin-A/C, junction plakoglobin, and PGAM1. Lastly, vimentin, nucleolin, and PGAM1 are verified with IHC. CONCLUSIONS AND CLINICAL RELEVANCE: The combination of MALDI-MSI, LC-MS/MS, and subsequent IHC furnishes a tool suitable for characterizing the molecular heterogeneity of tissue. It is also suited for use in identifying new representative biomarkers to enable a more individualized therapy.

Authors: Franziska Hoffmann, C. Umbreit, Thomas Krüger, D. Pelzel, G. Ernst, Olaf Kniemeyer, O. Guntinas-Lichius, A. Berndt, Ferdinand Von Eggeling

Date Published: 10th Nov 2018

Journal: Proteomics Clin Appl

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