C6 (E)

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Role of secreted Candida albicans proteins in immune evasion and pathogenicity

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

SEEK ID: https://funginet.hki-jena.de/projects/33

Public web page: http://www.funginet.de/project-c6.html

Organisms: Candida albicans

INF PALs: No PALs for this Project

Project created: 21st Jul 2015

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Showing 5 out of a possible 10 People Advanced People list for this Project with search and filtering

Niklas Beyersdorf

Projects: FungiNet C - Candida projects, FungiNet total, C6 (E)

Institutions: Julius Maximilians University Würzburg, Institute for Virology and Immunobiology

Prasad Dasari : inactive since 2019-10-25

Projects: C6 (E), FungiNet C - Candida projects, FungiNet total

Institutions: Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Peter Großmann

: inactive since 2020-06-01

Projects: FungiNet total, INF, A1, A2, A3, A4 (E), A5, A6, B1, B3 (E), B4, B5, C1, C2, C3, C4 (E), C5, C6 (E), Z1, Z2, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, B2

Institutions: Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

https://orcid.org/0000-0001-6106-724X

Expertise: Metabolic networks, Transcriptional Regulation, Bioinformatics, Integrative Data Analysis, Machine Learning, DNA supercoiling

Tools: LaTeX, Matlab, R, Systems biological modelling

Sarah Irmscher

Projects: C6 (E), FungiNet C - Candida projects, FungiNet total

Institutions: Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Naile Koleci

Projects: C6 (E)

Institutions: Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

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HITS Heidelberger Institut für Theoretische Studien gGmbH

Country: Germany

City: Heidelberg

Web page: https://www.h-its.org

Julius Maximilians University Würzburg, Institute for Virology and Immunobiology

Country: Germany

City: 97078 Würzburg

Web page: Not specified

Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Country: Germany

City: 07745 Jena

Web page: http://www.hki-jena.de

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Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic.

FungiNet C - Candida projects, C6 (E)

Abstract (Expand)

The PspC and Hic proteins of Streptococcus pneumoniae are some of the most variable microbial immune evasion proteins identified to date. Due to structural similarities and conserved binding profiles, …

Authors: S. Du, C. Vilhena, S. King, A. Sahagun-Ruiz, S. Hammerschmidt, C. Skerka, P. F. Zipfel

Date Published: 18th Jan 2021

Publication Type: Not specified

PubMed ID: 33462258

Citation: Sci Rep. 2021 Jan 18;11(1):1701. doi: 10.1038/s41598-020-79362-3.

Created: 19th Feb 2021 at 08:51, Last updated: 17th Jan 2024 at 10:24

Human Neutrophils Produce Antifungal Extracellular Vesicles against Aspergillus fumigatus.

A1, B4, C6 (E), Z2

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Abstract (Expand)

Polymorphonuclear granulocytes (PMNs) are indispensable for controlling life-threatening fungal infections. In addition to various effector mechanisms, PMNs also produce extracellular vesicles (EVs). … Their contribution to antifungal defense has remained unexplored. We reveal that the clinically important human-pathogenic fungus Aspergillus fumigatus triggers PMNs to release a distinct set of antifungal EVs (afEVs). Proteome analyses indicated that afEVs are enriched in antimicrobial proteins. The cargo and the release kinetics of EVs are modulated by the fungal strain confronted. Tracking of afEVs indicated that they associated with fungal cells and even entered fungal hyphae, resulting in alterations in the morphology of the fungal cell wall and dose-dependent antifungal effects. To assess as a proof of concept whether the antimicrobial proteins found in afEVs might contribute to growth inhibition of hyphae when present in the fungal cytoplasm, two human proteins enriched in afEVs, cathepsin G and azurocidin, were heterologously expressed in fungal hyphae. This led to reduced fungal growth relative to that of a control strain producing the human retinol binding protein 7. In conclusion, extracellular vesicles produced by neutrophils in response to A. fumigatus infection are able to associate with the fungus, limit growth, and elicit cell damage by delivering antifungal cargo. This finding offers an intriguing, previously overlooked mechanism of antifungal defense against A. fumigatus IMPORTANCE Invasive fungal infections caused by the mold Aspergillus fumigatus are a growing concern in the clinic due to the increasing use of immunosuppressive therapies and increasing antifungal drug resistance. These infections result in high rates of mortality, as treatment and diagnostic options remain limited. In healthy individuals, neutrophilic granulocytes are critical for elimination of A. fumigatus from the host; however, the exact extracellular mechanism of neutrophil-mediated antifungal activity remains unresolved. Here, we present a mode of antifungal defense employed by human neutrophils against A. fumigatus not previously described. We found that extracellular vesicles produced by neutrophils in response to A. fumigatus infection are able to associate with the fungus, limit growth, and elicit cell damage by delivering antifungal cargo. In the end, antifungal extracellular vesicle biology provides a significant step forward in our understanding of A. fumigatus host pathogenesis and opens up novel diagnostic and therapeutic possibilities.

Authors: I. A. Shopova, I. Belyaev, P. Dasari, S. Jahreis, M. C. Stroe, Z. Cseresnyes, A. K. Zimmermann, A. Medyukhina, C. M. Svensson, T. Kruger, V. Szeifert, S. Nietzsche, T. Conrad, M. G. Blango, O. Kniemeyer, M. von Lilienfeld-Toal, P. F. Zipfel, E. Ligeti, M. T. Figge, A. A. Brakhage

Date Published: 14th Apr 2020

Publication Type: Not specified

PubMed ID: 32291301

Citation: mBio. 2020 Apr 14;11(2). pii: mBio.00596-20. doi: 10.1128/mBio.00596-20.

Created: 15th Feb 2021 at 09:34, Last updated: 17th Jan 2024 at 10:24

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