Thomas Krüger

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About Thomas Krüger:

No description specified

SEEK ID: https://funginet.hki-jena.de/people/92

Location: Germany

ORCID: Not specified

Joined: 17th Nov 2016

Expertise: Host-Pathogen Interaction, Proteomics, Human-Pathogenic Fungi, Cyanobacterial Toxins

Tools: Liquid Chromatography, Mass spectrometry, Analytical Chemistry

Their tags

Aspergillus fumigatus Candida albicans Candida glabrata Lichtheimia corymbifera Posttranslational Modifications Proteomics

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

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FungiNet total

No description specified

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/home.html

Organisms: Aspergillus fumigatus, Candida albicans

In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-z2.html

Organisms: Aspergillus fumigatus, Candida albicans

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Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Country: Germany

City: 07745 Jena

Web page: http://www.hki-jena.de

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Showing 5 out of a possible 12 Publications Advanced Publications list for this Person with search and filtering

Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome.

INF, A1, Z2

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The RNA interference (RNAi) pathway has evolved numerous functionalities in eukaryotes, with many on display in Kingdom Fungi. RNAi can regulate gene expression, facilitate drug resistance, or even be …

Authors: A. A. Kelani, A. Bruch, F. Rivieccio, C. Visser, T. Kruger, D. Weaver, X. Pan, S. Schauble, G. Panagiotou, O. Kniemeyer, M. J. Bromley, P. Bowyer, A. E. Barber, A. A. Brakhage, M. G. Blango

Date Published: 19th Jun 2023

Publication Type: Journal

PubMed ID: 37019633

Citation: RNA. 2023 Jul;29(7):1033-1050. doi: 10.1261/rna.079350.122. Epub 2023 Apr 5.

Created: 19th Jan 2024 at 12:12

Candida albicans SR-Like Protein Kinases Regulate Different Cellular Processes: Sky1 Is Involved in Control of Ion Homeostasis, While Sky2 Is Important for Dipeptide Utilization.

INF, C2, Z2, B5

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Protein kinases play a crucial role in regulating cellular processes such as growth, proliferation, environmental adaptation and stress responses. Serine-arginine (SR) protein kinases are highly conserved …

Authors: P. Brandt, F. Gerwien, L. Wagner, T. Kruger, B. Ramirez-Zavala, M. H. Mirhakkak, S. Schauble, O. Kniemeyer, G. Panagiotou, A. A. Brakhage, J. Morschhauser, S. Vylkova

Date Published: 23rd May 2022

Publication Type: Journal

PubMed ID: 35601106

Citation: Front Cell Infect Microbiol. 2022 May 6;12:850531. doi: 10.3389/fcimb.2022.850531. eCollection 2022.

Created: 19th Jan 2024 at 12:17

Carbon Catabolite Repression in Filamentous Fungi Is Regulated by Phosphorylation of the Transcription Factor CreA.

FungiNet total, A1, Z2

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Filamentous fungi of the genus Aspergillus are of particular interest for biotechnological applications due to their natural capacity to secrete carbohydrate-active enzymes (CAZy) that target plant … biomass. The presence of easily metabolizable sugars such as glucose, whose concentrations increase during plant biomass hydrolysis, results in the repression of CAZy-encoding genes in a process known as carbon catabolite repression (CCR), which is undesired for the purpose of large-scale enzyme production. To date, the C2H2 transcription factor CreA has been described as the major CC repressor in Aspergillus spp., although little is known about the role of posttranslational modifications in this process. In this work, phosphorylation sites were identified by mass spectrometry on Aspergillus nidulans CreA, and subsequently, the previously identified but uncharacterized site S262, the characterized site S319, and the newly identified sites S268 and T308 were chosen to be mutated to nonphosphorylatable residues before their effect on CCR was investigated. Sites S262, S268, and T308 are important for CreA protein accumulation and cellular localization, DNA binding, and repression of enzyme activities. In agreement with a previous study, site S319 was not important for several here-tested phenotypes but is key for CreA degradation and induction of enzyme activities. All sites were shown to be important for glycogen and trehalose metabolism. This study highlights the importance of CreA phosphorylation sites for the regulation of CCR. These sites are interesting targets for biotechnological strain engineering without the need to delete essential genes, which could result in undesired side effects.IMPORTANCE In filamentous fungi, the transcription factor CreA controls carbohydrate metabolism through the regulation of genes encoding enzymes required for the use of alternative carbon sources. In this work, phosphorylation sites were identified on Aspergillus nidulans CreA, and subsequently, the two newly identified sites S268 and T308, the previously identified but uncharacterized site S262, and the previously characterized site S319 were chosen to be mutated to nonphosphorylatable residues before their effect on CCR was characterized. Sites S262, S268, and T308 are important for CreA protein accumulation and cellular localization, DNA binding, and repression of enzyme activities. In agreement with a previous study, site S319 is not important for several here-tested phenotypes but is key for CreA degradation and induction of enzyme activities. This work characterized novel CreA phosphorylation sites under carbon catabolite-repressing conditions and showed that they are crucial for CreA protein turnover, control of carbohydrate utilization, and biotechnologically relevant enzyme production.

Authors: L. J. de Assis, L. P. Silva, O. Bayram, P. Dowling, O. Kniemeyer, T. Kruger, A. A. Brakhage, Y. Chen, L. Dong, K. Tan, K. H. Wong, L. N. A. Ries, G. H. Goldman

Date Published: 5th Jan 2021

Publication Type: Not specified

PubMed ID: 33402538

Citation: mBio. 2021 Jan 5;12(1). pii: mBio.03146-20. doi: 10.1128/mBio.03146-20.

Created: 15th Feb 2021 at 09:23, Last updated: 17th Jan 2024 at 10:24

Functional surface proteomic profiling reveals the host heat-shock protein A8 as a mediator of Lichtheimia corymbifera recognition by murine alveolar macrophages.

A1, A5, B4, Z2, A6

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Mucormycosis is an emergent, fatal fungal infection of humans and warm-blooded animals caused by species of the order Mucorales. Immune cells of the innate immune system serve as the first line of …

Authors: M. I. A. Hassan, J. M. Kruse, T. Kruger, H. M. Dahse, Z. Cseresnyes, M. G. Blango, H. Slevogt, F. Horhold, V. Ast, R. Konig, M. T. Figge, O. Kniemeyer, A. A. Brakhage, K. Voigt

Date Published: 26th Jun 2020

Publication Type: Not specified

PubMed ID: 32583550

Citation: Environ Microbiol. 2020 Sep;22(9):3722-3740. doi: 10.1111/1462-2920.15140. Epub 2020 Jul 21.

Created: 15th Feb 2021 at 10:04, Last updated: 17th Jan 2024 at 10:24

Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles.

C4 (E)

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Extracellular vesicles have an important function in cellular communication. Here, we show that human and mouse monocytes release TGF-beta1-transporting vesicles in response to the pathogenic fungus …

Authors: L. D. Halder, E. A. H. Jo, M. Z. Hasan, M. Ferreira-Gomes, T. Kruger, M. Westermann, D. I. Palme, G. Rambach, N. Beyersdorf, C. Speth, I. D. Jacobsen, O. Kniemeyer, B. Jungnickel, P. F. Zipfel, C. Skerka

Date Published: 11th May 2020

Publication Type: Not specified

PubMed ID: 32393780

Citation: Nat Commun. 2020 May 11;11(1):2331. doi: 10.1038/s41467-020-16241-5.

Created: 18th Feb 2021 at 11:05, Last updated: 17th Jan 2024 at 10:24

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