Kerstin Voigt

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About Kerstin Voigt:

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SEEK ID: https://funginet.hki-jena.de/people/106

Location: Germany

ORCID: https://orcid.org/0000-0002-1345-9991

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

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FungiNet total

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Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/home.html

Organisms: Aspergillus fumigatus, Candida albicans

Surface proteins of Lichtheimia corymbifera as virulence determinants

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: https://www.funginet.de/project-a6.html

Organisms: Lichtheimia corymbifera

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Friedrich Schiller University Jena, Institute of Microbiology, Dept. of Microbiology and Molecular Biology

Country: Germany

City: 07745 Jena

Web page: Not specified

Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Country: Germany

City: 07745 Jena

Web page: http://www.hki-jena.de

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Visualization of HsbA in murine and human Lichtheimia infection

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Submitter: Franziska Hoffmann

Investigation: Spatial Proteomics

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Created: 17th Feb 2021 at 10:01

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The impact of episporic modification of Lichtheimia corymbifera on virulence and interaction with phagocytes.

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Fungal infections caused by the ancient lineage Mucorales are emerging and increasingly reported in humans. Comprehensive surveys on promising attributes from a multitude of possible virulence factors …

Authors: M. I. A. Hassan, M. Keller, M. Hillger, U. Binder, S. Reuter, K. Herold, A. Telagathoti, H. M. Dahse, S. Wicht, N. Trinks, S. Nietzsche, T. Deckert-Gaudig, V. Deckert, R. Mrowka, U. Terpitz, H. Peter Saluz, K. Voigt

Date Published: 18th Feb 2021

Publication Type: Not specified

PubMed ID: 33598103

Citation: Comput Struct Biotechnol J. 2021 Jan 20;19:880-896. doi: 10.1016/j.csbj.2021.01.023. eCollection 2021.

Created: 19th Feb 2021 at 07:40, Last updated: 17th Jan 2024 at 10:24

Functional surface proteomic profiling reveals the host heat-shock protein A8 as a mediator of Lichtheimia corymbifera recognition by murine alveolar macrophages.

A1, A5, B4, Z2, A6

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Mucormycosis is an emergent, fatal fungal infection of humans and warm-blooded animals caused by species of the order Mucorales. Immune cells of the innate immune system serve as the first line of …

Authors: M. I. A. Hassan, J. M. Kruse, T. Kruger, H. M. Dahse, Z. Cseresnyes, M. G. Blango, H. Slevogt, F. Horhold, V. Ast, R. Konig, M. T. Figge, O. Kniemeyer, A. A. Brakhage, K. Voigt

Date Published: 26th Jun 2020

Publication Type: Not specified

PubMed ID: 32583550

Citation: Environ Microbiol. 2020 Sep;22(9):3722-3740. doi: 10.1111/1462-2920.15140. Epub 2020 Jul 21.

Created: 15th Feb 2021 at 10:04, Last updated: 17th Jan 2024 at 10:24

Quantitative Impact of Cell Membrane Fluorescence Labeling on Phagocytosis Measurements in Confrontation Assays.

FungiNet A - Aspergillus projects, B4

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Phagocytosis is series of steps where the pathogens and the immune cells interact during an invasion. This starts with the adhesion process between the host and pathogen cells, and is followed by the … engulfment of the pathogens. Many analytical methods that are applied to characterize phagocytosis based on imaging the host-pathogen confrontation assays rely on the fluorescence labeling of cells. However, the potential effect of the membrane labeling on the quantitative results of the confrontation assays has not been studied in detail. In this study, we determine whether the fluorescence labeling processes themselves influence the results of the phagocytosis measurements. Here, alveolar macrophages, which form one of the most important compartments of the innate immune system, were used as an example of host cells, whereas Aspergillus fumigatus and Lichtheimia corymbifera that cause aspergillosis and mucormycosis, respectively, were studied as examples for pathogens. At first, our study investigated the importance of the sequence of steps of the fixation process when preparing the confrontation assay sample for microscopy studies. Here we showed that applying the fixation agent before the counter-staining causes miscalculations during the determination of the phagocytic measures. Furthermore, we also found that staining the macrophages with various concentrations of DID, as a typical membrane label, in most cases altered the capability of macrophages to phagocytose FITC-stained A. fumigatus and L. corymbifera spores in comparison with unlabeled macrophages. This effect of the DID staining showed a differential character dependent upon the labeling status and the specific type of pathogen. Moreover, labeling the spores of A. fumigatus and L. corymbifera with FITC increased the phagocytic measures during confrontation with unlabeled macrophages when compared to label-free spores. Overall, our study confirms that the staining process itself may significantly manipulate the quantitative outcome of the confrontation assay. As a result of our study, we also developed a user-friendly image analysis tool that analyses confrontation assays both with and without fluorescence labeling of the host cells and of the pathogens. Our image analysis algorithm saves experimental work effort and time, provides more precise results when calculating the phagocytic measures, and delivers a convenient analysis tool for the biologists to monitor host-pathogen interactions as they happen without the artifacts that fluorescence labeling imposes on biological interactions.

Authors: Z. Cseresnyes, M. I. A. Hassan, H. M. Dahse, K. Voigt, M. T. Figge

Date Published: 26th Jun 2020

Publication Type: Not specified

PubMed ID: 32582113

Citation: Front Microbiol. 2020 Jun 5;11:1193. doi: 10.3389/fmicb.2020.01193. eCollection 2020.

Created: 16th Feb 2021 at 15:46, Last updated: 17th Jan 2024 at 10:24

The geographical region of origin determines the phagocytic vulnerability of Lichtheimia strains.

FungiNet A - Aspergillus projects, B4

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Mucormycoses are life-threatening infections that affect patients suffering from immune deficiencies. We performed phagocytosis assays confronting various strains of Lichtheimia species with alveolar …

Authors: M. I. A. Hassan, Z. Cseresnyes, N. Al-Zaben, H. M. Dahse, R. J. Vilela de Oliveira, G. Walther, K. Voigt, M. T. Figge

Date Published: 23rd Jul 2019

Publication Type: Not specified

PubMed ID: 31330072

Citation: Environ Microbiol. 2019 Dec;21(12):4563-4581. doi: 10.1111/1462-2920.14752. Epub 2019 Aug 5.

Created: 16th Feb 2021 at 15:56, Last updated: 17th Jan 2024 at 10:24

Comparative Study on Alternative Splicing in Human Fungal Pathogens Suggests Its Involvement During Host Invasion.

INF, B1

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Alternative splicing (AS) is an important regulatory mechanism in eukaryotes but only little is known about its impact in fungi. Human fungal pathogens are of high clinical interest causing recurrent …

Authors: P. Sieber, K. Voigt, P. Kammer, S. Brunke, S. Schuster, J. Linde

Date Published: 19th Oct 2018

Publication Type: Not specified

PubMed ID: 30333805

Citation: Front Microbiol. 2018 Oct 2;9:2313. doi: 10.3389/fmicb.2018.02313. eCollection 2018.

Created: 11th Feb 2021 at 09:23, Last updated: 17th Jan 2024 at 10:24

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