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Daniela Albrecht-Eckardt

About Daniela Albrecht-Eckardt:
No description specified

SEEK ID: https://funginet.hki-jena.de/people/37

Location:  Germany

Expertise: Statistics, Data Management, Integrative Data Analysis, Bioinformatics, Systems Biology

Tools: Not specified

ORCID: Not specified

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Project positions

INF (BioControl Jena GmbH) ; Z2 (BioControl Jena GmbH)

Related items

Z2

In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-z2.html

INF

Integrated database for experimental data

Within the Collaborative Research Center / Transregio 124 “Pathogenic fungi and their human host: Networks of Interaction – FungiNet”, the INF project is responsible to analyse and share OMICS data. Databases help to foster the collaboration within FungiNet as an important point of the Collaborative Research Center. Therefore, we set-up two databases:

a) SEEK is a web-based platform to find, share and exchange unstructured data and knowledge, such as
...

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-inf.html

BioControl Jena GmbH

Country:  Germany

City: 07745 Jena

Web page: httP://www.biocontrol-jena.com

Gene regulation by hapX
Z2

P Hortschansky, M Eisendle, Q Al-Abdallah, A D Schmidt, S Bergmann, M Thön, O Kniemeyer, B Abt, B Seeber, E R Werner, M Kato, A A Brakhage and H Haas. Interaction of HapX with the CCAAT-binding complex-a novel mechanism of gene regulation by iron. EMBO J. 2007 Jul 11;26(13):3157-68. Epub 2007 Jun 14

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Farnesol
Z2

Wartenberg D, Vödisch M, Kniemeyer O, Albrecht-Eckardt D, Scherlach K, Winkler R, Weide M, Brakhage AA. Proteome analysis of the farnesol-induced stress response in Aspergillus nidulans-The role of a putative dehydrin. J Proteomics. 2012 May 23.

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Temperature Shift Proteomic
Z2

D Albrecht, R Guthke, A A Brakhage, O Kniemeyer. Integrative analysis of the heat shock response in Aspergillus fumigatus. BMC Genomics. 2010 Jan 15;11:32.

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Find corresponding transcriptomic data at ArrayExpress: E-MEXP-332

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Secretome AMM/72 h
Z2

Dirk Wartenberg, Katrin Lapp, Ilse D. Jacobsen, Hans-Martin Dahse, Olaf Kniemeyer, Thorsten Heinekamp, Axel A. Brakhage
Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knoell-Institute (HKI), Jena, Germany. Secretome analysis of Aspergillus fumigatus reveals Asp-hemolysin as a major secreted protein. Int J Med Microbiol. 2011, Nov;301(7):602-11

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Proteomic profiling of serological responses to A.fum antigens (IA)
Z2

Teutschbein J, Simon S, Lother J, Springer J, Hortschansky P, Morton CO, Löffler J, Einsele H, Conneally E, Rogers TR, Guthke R, Brakhage AA, Kniemeyer O. Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis. J Proteome Res 2016 Mar 24

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9 hidden items
deltaHapX
Z2
No description specified

Person responsible: Daniela Albrecht-Eckardt

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wt
Z2
No description specified

Person responsible: Daniela Albrecht-Eckardt

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Internal Standard
Z2

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Person responsible: Daniela Albrecht-Eckardt

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Farnesol Treatment
Z2

Aspergillus nidulans R21 was grown over night in AMM at 37°C and 200 rpm. The culture was treated with 50 µM farnesol or the same volume of ethanol without farnesol as control. The culture was harvested after 3 hours of further incubation.

Person responsible: Daniela Albrecht-Eckardt

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Control
Z2
No description specified

Person responsible: Daniela Albrecht-Eckardt

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20 hidden items
48h
Z2
No description specified

Contributor: Daniela Albrecht-Eckardt

Assay type: Experimental Assay Type

Technology type: Technology Type

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deltaHapX
Z2
No description specified

Contributor: Daniela Albrecht-Eckardt

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

wt
Z2
No description specified

Contributor: Daniela Albrecht-Eckardt

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Standard gels
Z2
No description specified

Contributor: Daniela Albrecht-Eckardt

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Farnesol gels
Z2
No description specified

Contributor: Daniela Albrecht-Eckardt

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

20 hidden items
deltaHapX data
Z2
No description specified

Creator: Daniela Albrecht-Eckardt

Contributor: Daniela Albrecht-Eckardt

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wt data
Z2
No description specified

Creator: Daniela Albrecht-Eckardt

Contributor: Daniela Albrecht-Eckardt

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Standard data
Z2
No description specified

Creator: Daniela Albrecht-Eckardt

Contributor: Daniela Albrecht-Eckardt

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Farnesol data
Z2
No description specified

Creator: Daniela Albrecht-Eckardt

Contributor: Daniela Albrecht-Eckardt

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Control data
Z2
No description specified

Creator: Daniela Albrecht-Eckardt

Contributor: Daniela Albrecht-Eckardt

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20 hidden items
1 hidden item
Ahr1 and Tup1 Contribute to the Transcriptional Control of Virulence-Associated Genes in Candida albicans.
A1, C1, C2, C3, C5, Z2

Abstract (Expand)

The capacity of Candida albicans to reversibly change its morphology between yeast and filamentous stages is crucial for its virulence. Formation of hyphae correlates with the upregulation of genes … ALS3 and ECE1, which are involved in pathogenicity processes such as invasion, iron acquisition, and host cell damage. The global repressor Tup1 and its cofactor Nrg1 are considered to be the main antagonists of hyphal development in C. albicans However, our experiments revealed that Tup1, but not Nrg1, was required for full expression of ALS3 and ECE1 In contrast to NRG1, overexpression of TUP1 was found to inhibit neither filamentous growth nor transcription of ALS3 and ECE1 In addition, we identified the transcription factor Ahr1 as being required for full expression of both genes. A hyperactive version of Ahr1 bound directly to the promoters of ALS3 and ECE1 and induced their transcription even in the absence of environmental stimuli. This regulation worked even in the absence of the crucial hyphal growth regulators Cph1 and Efg1 but was dependent on the presence of Tup1. Overall, our results show that Ahr1 and Tup1 are key contributors in the complex regulation of virulence-associated genes in the different C. albicans morphologies.IMPORTANCE Candida albicans is a major human fungal pathogen and the leading cause of systemic Candida infections. In recent years, Als3 and Ece1 were identified as important factors for fungal virulence. Transcription of both corresponding genes is closely associated with hyphal growth. Here, we describe how Tup1, normally a global repressor of gene expression as well as of filamentation, and the transcription factor Ahr1 contribute to full expression of ALS3 and ECE1 in C. albicans hyphae. Both regulators are required for high mRNA amounts of the two genes to ensure functional relevant protein synthesis and localization. These observations identified a new aspect of regulation in the complex transcriptional control of virulence-associated genes in C. albicans.

Authors: S. Ruben, E. Garbe, Selene Mogavero, Daniela Albrecht-Eckardt, D. Hellwig, A. Hader, Thomas Krüger, K. Gerth, Ilse Jacobsen, O. Elshafee, S. Brunke, Kerstin Hünniger, Olaf Kniemeyer, Axel Brakhage, Joachim Morschhäuser, Bernhard Hube, Slavena Vylkova, Oliver Kurzai, R. Martin

Date Published: 28th Apr 2020

Journal: mBio

Candida albicans Induces Metabolic Reprogramming in Human NK Cells and Responds to Perforin with a Zinc Depletion Response
FungiNet A - Aspergillus projects, FungiNet C - Candida projects

Abstract (Expand)

As part of the innate immune system, natural killer (NK) cells are directly involved in the response to fungal infections. Perforin has been identified as the major effector molecule acting against … many fungal pathogens. While several studies have shown that perforin mediated fungicidal effects can contribute to fungal clearance, neither the activation of NK cells by fungal pathogens nor the effects of perforin on fungal cells are well-understood. In a dual approach, we have studied the global gene expression pattern of primary and cytokine activated NK cells after co-incubation with Candida albicans and the transcriptomic adaptation of C. albicans to perforin exposure. NK cells responded to the fungal pathogen with an up-regulation of genes involved in immune signaling and release of cytokines. Furthermore, we observed a pronounced increase of genes involved in glycolysis and glycolysis inhibitor 2-deoxy-D-glucose impaired C. albicans induced NK cell activation. This strongly indicates that metabolic adaptation is a major part of the NK cell response to C. albicans infections. In the fungal pathogen, perforin induced a strong up-regulation of several fungal genes involved in the zinc depletion response, such as PRA1 and ZRT1. These data suggest that fungal zinc homeostasis is linked to the reaction to perforin secreted by NK cells. However, deletion mutants in PRA1 and ZRT1 did not show altered susceptibility to perforin.

Authors: Dragana Slavkovic Lukic, J. Voigt, M. Bouzani, Jürgen Löffler, Daniela Albrecht-Eckardt, Michael Weber, Stefanie Allert, R. Martin, Oliver Kurzai, Kerstin Hünniger

Date Published: 19th May 2016

Journal: Front Microbiol

Candida albicans infection leads to barrier breakdown and a MAPK/NF-kappaB mediated stress response in the intestinal epithelial cell line C2BBe1
FungiNet B - Bioinformatics projects, INF

Abstract (Expand)

Intestinal epithelial cells (IEC) form a tight barrier to the gut lumen. Paracellular permeability of the intestinal barrier is regulated by tight junction proteins and can be modulated by microorganisms … and other stimuli. The polymorphic fungus Candida albicans, a frequent commensal of the human mucosa has the capacity of traversing this barrier and establishing systemic disease within the host. Infection of polarized C2BBe1 IEC with wild-type C. albicans led to a transient increase of transepithelial electric resistance (TEER) before subsequent barrier disruption, accompanied by a strong decline of junctional protein levels and substantial, but considerably delayed cytotoxicity. Time-resolved microarray-based transcriptome analysis of C. albicans challenged IEC revealed a prominent role of NF-kappaB and MAPK signaling pathways in the response to infection. Hence, we inferred a gene regulatory network based on differentially expressed NF-kappaB and MAPK pathway components and their predicted transcriptional targets. The network model predicted activation of GDF15 by NF-kappaB was experimentally validated. Furthermore, inhibition of NF-kappaB activation in C. albicans infected C2BBe1 cells led to enhanced cytotoxicity in the epithelial cells. Taken together our study identifies NF-kappaB activation as an important protective signaling pathway in the response of epithelial cells to C. albicans. This article is protected by copyright. All rights reserved.

Authors: M. Bohringer, S. Pohlers, Sylvie Schulze, Daniela Albrecht-Eckardt, J. Piegsa, M. Weber, R. Martin, Kerstin Hünniger, Jörg Linde, Reinhard Guthke, Oliver Kurzai

Date Published: 12th Jan 2016

Journal: Cell Microbiol

A core filamentation response network in Candida albicans is restricted to eight genes
FungiNet C - Candida projects

Abstract (Expand)

Although morphological plasticity is a central virulence trait of Candida albicans, the number of filament-associated genes and the interplay of mechanisms regulating their expression remain unknown. … By correlation-based network modeling of the transcriptional response to different defined external stimuli for morphogenesis we identified a set of eight genes with highly correlated expression patterns, forming a core filamentation response. This group of genes included ALS3, ECE1, HGT2, HWP1, IHD1 and RBT1 which are known or supposed to encode for cell- wall associated proteins as well as the Rac1 guanine nucleotide exchange factor encoding gene DCK1 and the unknown function open reading frame orf19.2457. The validity of network modeling was confirmed using a dataset of advanced complexity that describes the transcriptional response of C. albicans during epithelial invasion as well as comparing our results with other previously published transcriptome studies. Although the set of core filamentation response genes was quite small, several transcriptional regulators are involved in the control of their expression, depending on the environmental condition.

Authors: R. Martin, Daniela Albrecht-Eckardt, S. Brunke, Bernhard Hube, Kerstin Hünniger, Oliver Kurzai

Date Published: 14th Mar 2013

Journal: PLoS One

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