FungiNet A - Aspergillus projects

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Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

SEEK ID: https://funginet.hki-jena.de/projects/15

Public web page: http://www.funginet.de/project-area-a.html

Organisms: Aspergillus fumigatus

INF PALs: No PALs for this Project

Project created: 1st Oct 2012

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Showing 5 out of a possible 34 People Advanced People list for this Project with search and filtering

Tanja Bedke

Projects: FungiNet A - Aspergillus projects, FungiNet total

Institutions: Julius Maximilians University Würzburg, Department of Internal Medicine II

Andreas Beilhack

Projects: FungiNet total, FungiNet A - Aspergillus projects, A3

Institutions: Julius Maximilians University Würzburg, Department of Internal Medicine II

Expertise: Immunology, Hematopoietic cell transplantation, Inflammation, Imaging, Microscopy

Tools: Multiparameter-Flowcytometry, Bioluminescence Imaging, Laser-scanning-fluorescence microscopy, Intravital microscopy, Mouse models

Carsten Berges

Projects: FungiNet A - Aspergillus projects, FungiNet total, A4 (E)

Institutions: Julius Maximilians University Würzburg, Department of Internal Medicine II

Axel Brakhage

Projects: FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, FungiNet total, A1, Z1

Institutions: Friedrich Schiller University Jena, Institute of Microbiology, Dept. of Microbiology and Molecular Biology, Friedrich Schiller University Jena, Jena School for Microbial Communication, Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Matthias Drobny

Projects: A2, FungiNet A - Aspergillus projects, FungiNet total

Institutions: Julius Maximilians University Würzburg, Department of Internal Medicine II

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

Showing 5 out of a possible 18 Institutions Advanced Institutions list for this Project with search and filtering

Friedrich Schiller University Jena, Center for Innovation Competence (ZIK) Septomics

Country: Germany

City: 07745 Jena

Web page: Not specified

Friedrich Schiller University Jena, Institute of Nutrition

Country: Germany

City: 07743 Jena

Web page: Not specified

Friedrich Schiller University Jena, Jena School for Microbial Communication

Country: Germany

City: 07743 Jena

Web page: Not specified

Friedrich Schiller University Jena, Bioinformatics/High Throughput Analysis, Faculty of Mathematics und Computer Science

Country: Germany

City: 07743 Jena

Web page: Not specified

Friedrich Schiller University Jena, Chair MIcrobial Pathogenicity

Country: Germany

City: 07745 Jena

Web page: Not specified

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DMykG_2015 Programmheft

FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, Z2

No description specified

Creator: Reinhard Guthke

Submitter: Reinhard Guthke

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 4th Mar 2016 at 16:00, Last updated: 7th Jun 2018 at 15:41

DMykG 2015 Programmheft

FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, Z2

No description specified

Creator: Reinhard Guthke

Submitter: Reinhard Guthke

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 4th Mar 2016 at 16:02, Last updated: 7th Jun 2018 at 15:41

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Showing 5 out of a possible 34 Publications Advanced Publications list for this Project with search and filtering

N-Heterocyclization in Gliotoxin Biosynthesis is Catalyzed by a Distinct Cytochrome P450 Monooxygenase.

FungiNet A - Aspergillus projects

Abstract (Expand)

Gliotoxin and related epidithiodiketopiperazines (ETP) from diverse fungi feature highly functionalized hydroindole scaffolds with an array of medicinally and ecologically relevant activities. Mutation …

Authors: D. H. Scharf, P. Chankhamjon, K. Scherlach, J. Dworschak, T. Heinekamp, M. Roth, A. A. Brakhage, C. Hertweck

Date Published: 15th Jan 2021

Publication Type: Not specified

PubMed ID: 32835438

Citation: Chembiochem. 2021 Jan 15;22(2):336-339. doi: 10.1002/cbic.202000550. Epub 2020 Oct 5.

Created: 18th Feb 2021 at 12:53, Last updated: 17th Jan 2024 at 10:24

Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections.

FungiNet A - Aspergillus projects, B4

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Abstract (Expand)

Lipid rafts form signaling platforms on biological membranes with incompletely characterized role in immune response to infection. Here we report that lipid-raft microdomains are essential components …

Authors: F. Schmidt, A. Thywissen, M. Goldmann, C. Cunha, Z. Cseresnyes, H. Schmidt, M. Rafiq, S. Galiani, M. H. Graler, G. Chamilos, J. F. Lacerda, A. Jr Campos, C. Eggeling, M. T. Figge, T. Heinekamp, S. G. Filler, A. Carvalho, A. A. Brakhage

Date Published: 18th Aug 2020

Publication Type: Not specified

PubMed ID: 32814035

Citation: Cell Rep. 2020 Aug 18;32(7):108017. doi: 10.1016/j.celrep.2020.108017.

Created: 16th Feb 2021 at 16:12, Last updated: 17th Jan 2024 at 10:24

Quantitative Impact of Cell Membrane Fluorescence Labeling on Phagocytosis Measurements in Confrontation Assays.

FungiNet A - Aspergillus projects, B4

Abstract (Expand)

Phagocytosis is series of steps where the pathogens and the immune cells interact during an invasion. This starts with the adhesion process between the host and pathogen cells, and is followed by the … engulfment of the pathogens. Many analytical methods that are applied to characterize phagocytosis based on imaging the host-pathogen confrontation assays rely on the fluorescence labeling of cells. However, the potential effect of the membrane labeling on the quantitative results of the confrontation assays has not been studied in detail. In this study, we determine whether the fluorescence labeling processes themselves influence the results of the phagocytosis measurements. Here, alveolar macrophages, which form one of the most important compartments of the innate immune system, were used as an example of host cells, whereas Aspergillus fumigatus and Lichtheimia corymbifera that cause aspergillosis and mucormycosis, respectively, were studied as examples for pathogens. At first, our study investigated the importance of the sequence of steps of the fixation process when preparing the confrontation assay sample for microscopy studies. Here we showed that applying the fixation agent before the counter-staining causes miscalculations during the determination of the phagocytic measures. Furthermore, we also found that staining the macrophages with various concentrations of DID, as a typical membrane label, in most cases altered the capability of macrophages to phagocytose FITC-stained A. fumigatus and L. corymbifera spores in comparison with unlabeled macrophages. This effect of the DID staining showed a differential character dependent upon the labeling status and the specific type of pathogen. Moreover, labeling the spores of A. fumigatus and L. corymbifera with FITC increased the phagocytic measures during confrontation with unlabeled macrophages when compared to label-free spores. Overall, our study confirms that the staining process itself may significantly manipulate the quantitative outcome of the confrontation assay. As a result of our study, we also developed a user-friendly image analysis tool that analyses confrontation assays both with and without fluorescence labeling of the host cells and of the pathogens. Our image analysis algorithm saves experimental work effort and time, provides more precise results when calculating the phagocytic measures, and delivers a convenient analysis tool for the biologists to monitor host-pathogen interactions as they happen without the artifacts that fluorescence labeling imposes on biological interactions.

Authors: Z. Cseresnyes, M. I. A. Hassan, H. M. Dahse, K. Voigt, M. T. Figge

Date Published: 26th Jun 2020

Publication Type: Not specified

PubMed ID: 32582113

Citation: Front Microbiol. 2020 Jun 5;11:1193. doi: 10.3389/fmicb.2020.01193. eCollection 2020.

Created: 16th Feb 2021 at 15:46, Last updated: 17th Jan 2024 at 10:24

Enolase From Aspergillus fumigatus Is a Moonlighting Protein That Binds the Human Plasma Complement Proteins Factor H, FHL-1, C4BP, and Plasminogen.

FungiNet A - Aspergillus projects, FungiNet C - Candida projects, B4

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Abstract (Expand)

The opportunistic fungal pathogen Aspergillus fumigatus can cause severe infections, particularly in immunocompromised individuals. Upon infection, A. fumigatus faces the powerful and directly acting …

Authors: P. Dasari, N. Koleci, I. A. Shopova, D. Wartenberg, N. Beyersdorf, S. Dietrich, A. Sahagun-Ruiz, M. T. Figge, C. Skerka, A. A. Brakhage, P. F. Zipfel

Date Published: 12th Dec 2019

Publication Type: Not specified

PubMed ID: 31824478

Citation: Front Immunol. 2019 Nov 22;10:2573. doi: 10.3389/fimmu.2019.02573. eCollection 2019.

Created: 16th Feb 2021 at 15:54, Last updated: 17th Jan 2024 at 10:24

The geographical region of origin determines the phagocytic vulnerability of Lichtheimia strains.

FungiNet A - Aspergillus projects, B4

Abstract (Expand)

Mucormycoses are life-threatening infections that affect patients suffering from immune deficiencies. We performed phagocytosis assays confronting various strains of Lichtheimia species with alveolar …

Authors: M. I. A. Hassan, Z. Cseresnyes, N. Al-Zaben, H. M. Dahse, R. J. Vilela de Oliveira, G. Walther, K. Voigt, M. T. Figge

Date Published: 23rd Jul 2019

Publication Type: Not specified

PubMed ID: 31330072

Citation: Environ Microbiol. 2019 Dec;21(12):4563-4581. doi: 10.1111/1462-2920.14752. Epub 2019 Aug 5.

Created: 16th Feb 2021 at 15:56, Last updated: 17th Jan 2024 at 10:24

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On the role of CEACAM receptors during systemic candidiasis

FungiNet total, FungiNet A - Aspergillus projects, A5