Carl-Magnus Svensson

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About Carl-Magnus Svensson:

No description specified

SEEK ID: https://funginet.hki-jena.de/people/124

Location: Germany

ORCID: Not specified

Joined: 10th Feb 2021

Expertise: Not specified

Tools: Not specified

Their tags

Antibiotic susceptibility Aspergillus fumigatus Automated image analysis Bayesian inference Biofilm Candida albicans Candida glabrata candidalysin Cell track analysis Cell tracking Clustering Complement system Confrontation assay Droplets Escherichia coli flotillin host cell damage host cell invasion ImageJ Immune evasion intestinal barrier Lactobacilli Lichtheimia corymbifera Machine Learning Macrophages Microbiota Microfluidics Mouse model NADPH oxidase necrosis Organ-on-chip Ornstein-Uhlenbeck process PMN Python Review Sepsis Somatic hypermutation Streptococcus pneumoniae translocation

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

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Image data analysis and agent-based modelling of the spatio-temporal interaction between immune cells and human-pathogenic fungi

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-b4.html

Organisms: Aspergillus fumigatus, Candida albicans

FungiNet C - Candida projects

No description specified

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-area-c.html

Organisms: Candida albicans

FungiNet A - Aspergillus projects

No description specified

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-area-a.html

Organisms: Aspergillus fumigatus

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Leibniz-Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)

Country: Germany

City: 07745 Jena

Web page: http://www.hki-jena.de

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Cell track generator

README

This package contains code that generates and analyses tracks in 2D given three distinct condition; non-persistent, persistent and directed by the Ornstein-Uhlenbeck process. Full details on the analysis can be found in the article "Svensson et al., Untangling cell tracks: quantifying cell migration by time lapse image data analysis, Cytometry Pt A, 2017". The paper can be found on ...

Creator: Carl-Magnus Svensson

Submitter: Carl-Magnus Svensson

Model type: Ordinary differential equations (ODE)

Model format: Not specified

Environment: Not specified

Organism: Not specified

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 19th Feb 2021 at 09:06

ACAQ3

This is the image analysis algorithm used in Cseresnyes, Kraibooj and Figge, Hessian-based quantitative image analysis of host-pathogen confrontation assays Cytometry A. 2018 Mar;93(3):346-356. doi: 10.1002/cyto.a.23201. Code is being maintained at https://github.com/applied-systems-biology/ACAQ3.

Read.me

This package contains code that implements the Hessian-based macrophage segmentation algorithm. In addition, the ...

Creator: Carl-Magnus Svensson

Submitter: Carl-Magnus Svensson

Model type: Not specified

Model format: Not specified

Environment: Not specified

Organism: Aspergillus fumigatus

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 16th Feb 2021 at 15:44, Last updated: 16th Feb 2021 at 15:44

Bayesian analysis of pseudo-time resolved somatic hyper-mutations

Code for Bayesian analysis of pseudo-time resolved somatic hyper-mutations. Hirth et al., "Regulation of the germinal center reaction and somatic hypermutation dynamics by homologus recombination", Jounal of Immunology. 9, 2019, ji1900483; DOI: https://doi.org/10.4049/jimmunol.1900483

README

This package contains code and data that calulates and tests differences in realative mutation frequancy between Control and ...

Creator: Carl-Magnus Svensson

Submitter: Carl-Magnus Svensson

Model type: Not specified

Model format: Not specified

Environment: Not specified

Organism: Not specified

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 16th Feb 2021 at 10:42

Showing 5 out of a possible 16 Publications Advanced Publications list for this Person with search and filtering

Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections.

FungiNet A - Aspergillus projects, B4

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Abstract (Expand)

Lipid rafts form signaling platforms on biological membranes with incompletely characterized role in immune response to infection. Here we report that lipid-raft microdomains are essential components …

Authors: F. Schmidt, A. Thywissen, M. Goldmann, C. Cunha, Z. Cseresnyes, H. Schmidt, M. Rafiq, S. Galiani, M. H. Graler, G. Chamilos, J. F. Lacerda, A. Jr Campos, C. Eggeling, M. T. Figge, T. Heinekamp, S. G. Filler, A. Carvalho, A. A. Brakhage

Date Published: 18th Aug 2020

Publication Type: Not specified

PubMed ID: 32814035

Citation: Cell Rep. 2020 Aug 18;32(7):108017. doi: 10.1016/j.celrep.2020.108017.

Created: 16th Feb 2021 at 16:12, Last updated: 17th Jan 2024 at 10:24

Quantitative Impact of Cell Membrane Fluorescence Labeling on Phagocytosis Measurements in Confrontation Assays.

FungiNet A - Aspergillus projects, B4

Abstract (Expand)

Phagocytosis is series of steps where the pathogens and the immune cells interact during an invasion. This starts with the adhesion process between the host and pathogen cells, and is followed by the … engulfment of the pathogens. Many analytical methods that are applied to characterize phagocytosis based on imaging the host-pathogen confrontation assays rely on the fluorescence labeling of cells. However, the potential effect of the membrane labeling on the quantitative results of the confrontation assays has not been studied in detail. In this study, we determine whether the fluorescence labeling processes themselves influence the results of the phagocytosis measurements. Here, alveolar macrophages, which form one of the most important compartments of the innate immune system, were used as an example of host cells, whereas Aspergillus fumigatus and Lichtheimia corymbifera that cause aspergillosis and mucormycosis, respectively, were studied as examples for pathogens. At first, our study investigated the importance of the sequence of steps of the fixation process when preparing the confrontation assay sample for microscopy studies. Here we showed that applying the fixation agent before the counter-staining causes miscalculations during the determination of the phagocytic measures. Furthermore, we also found that staining the macrophages with various concentrations of DID, as a typical membrane label, in most cases altered the capability of macrophages to phagocytose FITC-stained A. fumigatus and L. corymbifera spores in comparison with unlabeled macrophages. This effect of the DID staining showed a differential character dependent upon the labeling status and the specific type of pathogen. Moreover, labeling the spores of A. fumigatus and L. corymbifera with FITC increased the phagocytic measures during confrontation with unlabeled macrophages when compared to label-free spores. Overall, our study confirms that the staining process itself may significantly manipulate the quantitative outcome of the confrontation assay. As a result of our study, we also developed a user-friendly image analysis tool that analyses confrontation assays both with and without fluorescence labeling of the host cells and of the pathogens. Our image analysis algorithm saves experimental work effort and time, provides more precise results when calculating the phagocytic measures, and delivers a convenient analysis tool for the biologists to monitor host-pathogen interactions as they happen without the artifacts that fluorescence labeling imposes on biological interactions.

Authors: Z. Cseresnyes, M. I. A. Hassan, H. M. Dahse, K. Voigt, M. T. Figge

Date Published: 26th Jun 2020

Publication Type: Not specified

PubMed ID: 32582113

Citation: Front Microbiol. 2020 Jun 5;11:1193. doi: 10.3389/fmicb.2020.01193. eCollection 2020.

Created: 16th Feb 2021 at 15:46, Last updated: 17th Jan 2024 at 10:24

The Transcription Factor Stp2 Is Important for Candida albicans Biofilm Establishment and Sustainability.

FungiNet C - Candida projects, B4

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Abstract (Expand)

The fungal pathogen Candida albicans forms polymorphic biofilms where hyphal morphogenesis and metabolic adaptation are tightly coordinated by a complex intertwined network of transcription factors. …

Authors: B. Bottcher, B. Hoffmann, E. Garbe, T. Weise, Z. Cseresnyes, P. Brandt, S. Dietrich, D. Driesch, M. T. Figge, S. Vylkova

Date Published: 20th May 2020

Publication Type: Not specified

PubMed ID: 32425915

Citation: Front Microbiol. 2020 Apr 30;11:794. doi: 10.3389/fmicb.2020.00794. eCollection 2020.

Created: 16th Feb 2021 at 15:20, Last updated: 17th Jan 2024 at 10:24

Human Neutrophils Produce Antifungal Extracellular Vesicles against Aspergillus fumigatus.

A1, B4, C6 (E), Z2

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Abstract (Expand)

Polymorphonuclear granulocytes (PMNs) are indispensable for controlling life-threatening fungal infections. In addition to various effector mechanisms, PMNs also produce extracellular vesicles (EVs). … Their contribution to antifungal defense has remained unexplored. We reveal that the clinically important human-pathogenic fungus Aspergillus fumigatus triggers PMNs to release a distinct set of antifungal EVs (afEVs). Proteome analyses indicated that afEVs are enriched in antimicrobial proteins. The cargo and the release kinetics of EVs are modulated by the fungal strain confronted. Tracking of afEVs indicated that they associated with fungal cells and even entered fungal hyphae, resulting in alterations in the morphology of the fungal cell wall and dose-dependent antifungal effects. To assess as a proof of concept whether the antimicrobial proteins found in afEVs might contribute to growth inhibition of hyphae when present in the fungal cytoplasm, two human proteins enriched in afEVs, cathepsin G and azurocidin, were heterologously expressed in fungal hyphae. This led to reduced fungal growth relative to that of a control strain producing the human retinol binding protein 7. In conclusion, extracellular vesicles produced by neutrophils in response to A. fumigatus infection are able to associate with the fungus, limit growth, and elicit cell damage by delivering antifungal cargo. This finding offers an intriguing, previously overlooked mechanism of antifungal defense against A. fumigatus IMPORTANCE Invasive fungal infections caused by the mold Aspergillus fumigatus are a growing concern in the clinic due to the increasing use of immunosuppressive therapies and increasing antifungal drug resistance. These infections result in high rates of mortality, as treatment and diagnostic options remain limited. In healthy individuals, neutrophilic granulocytes are critical for elimination of A. fumigatus from the host; however, the exact extracellular mechanism of neutrophil-mediated antifungal activity remains unresolved. Here, we present a mode of antifungal defense employed by human neutrophils against A. fumigatus not previously described. We found that extracellular vesicles produced by neutrophils in response to A. fumigatus infection are able to associate with the fungus, limit growth, and elicit cell damage by delivering antifungal cargo. In the end, antifungal extracellular vesicle biology provides a significant step forward in our understanding of A. fumigatus host pathogenesis and opens up novel diagnostic and therapeutic possibilities.

Authors: I. A. Shopova, I. Belyaev, P. Dasari, S. Jahreis, M. C. Stroe, Z. Cseresnyes, A. K. Zimmermann, A. Medyukhina, C. M. Svensson, T. Kruger, V. Szeifert, S. Nietzsche, T. Conrad, M. G. Blango, O. Kniemeyer, M. von Lilienfeld-Toal, P. F. Zipfel, E. Ligeti, M. T. Figge, A. A. Brakhage

Date Published: 14th Apr 2020

Publication Type: Not specified

PubMed ID: 32291301

Citation: mBio. 2020 Apr 14;11(2). pii: mBio.00596-20. doi: 10.1128/mBio.00596-20.

Created: 15th Feb 2021 at 09:34, Last updated: 17th Jan 2024 at 10:24

Enolase From Aspergillus fumigatus Is a Moonlighting Protein That Binds the Human Plasma Complement Proteins Factor H, FHL-1, C4BP, and Plasminogen.

FungiNet A - Aspergillus projects, FungiNet C - Candida projects, B4

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Abstract (Expand)

The opportunistic fungal pathogen Aspergillus fumigatus can cause severe infections, particularly in immunocompromised individuals. Upon infection, A. fumigatus faces the powerful and directly acting …

Authors: P. Dasari, N. Koleci, I. A. Shopova, D. Wartenberg, N. Beyersdorf, S. Dietrich, A. Sahagun-Ruiz, M. T. Figge, C. Skerka, A. A. Brakhage, P. F. Zipfel

Date Published: 12th Dec 2019

Publication Type: Not specified

PubMed ID: 31824478

Citation: Front Immunol. 2019 Nov 22;10:2573. doi: 10.3389/fimmu.2019.02573. eCollection 2019.

Created: 16th Feb 2021 at 15:54, Last updated: 17th Jan 2024 at 10:24

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