Microdissection-An Essential Prerequisite for Spatial Cancer Omics.
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BiBTeXThe problem with cancer tissue is that its intratumoral heterogeneity and its complexity is extremely high as cells possess, depending on their location and function, different mutations, different mRNA expression and the highest intricacy in the protein pattern. Prior to genomic and proteomic analyses, it is therefore indispensable to identify the exact part of the tissue or even the exact cell. Laser-based microdissection is a tried and tested technique able to produce pure and well-defined cell material for further analysis with proteomic and genomic techniques. It sheds light on the heterogeneity of cancer or other complex diseases and enables the identification of biomarkers. This review aims to raise awareness for the reconsideration of laser-based microdissection and seeks to present current state-of-the-art combinations with omic techniques.
SEEK ID: https://funginet.hki-jena.de/publications/167
PubMed ID: 32578340
Projects: Z2
Publication type: Not specified
Journal: Proteomics
Citation: Proteomics. 2020 Sep;20(17-18):e2000077. doi: 10.1002/pmic.202000077. Epub 2020 Jul 6.
Date Published: 25th Jun 2020
Registered Mode: Not specified
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Created: 17th Feb 2021 at 12:21
Last updated: 17th Jan 2024 at 10:24
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https://orcid.org/0000-0002-6872-924XI am responisble for the spatial proteomics part in Z2. We provide a proteomic platform to study spatial changes of the proteome during host-fungal pathogen interaction using MALDI-MS Imaging and Lasermicrodissection. As type of biological sample we can work with cryo and formalin fixed tissue, as well as cell cultures and lysed sample solutions. Please get into contact with me if you are interested in MALDI-MS Imaging for your research project.
The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...
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Web page: http://www.funginet.de/home.html
In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.
This service and research topic deals with the spatially resolved field of proteomics which allows targetet and untargeted host-pathogen interaction studies. Please get into contact with Franziska Hoffmann and Ferdinand von Eggeling if you are interested in spatial proteomics.
