Andreas Beilhack

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About Andreas Beilhack:

No description specified

SEEK ID: https://funginet.hki-jena.de/people/34

Location: Germany

ORCID: Not specified

Joined: 19th Dec 2012

Expertise: Immunology, Hematopoietic cell transplantation, Inflammation, Imaging, Microscopy

Tools: Multiparameter-Flowcytometry, Bioluminescence Imaging, Laser-scanning-fluorescence microscopy, Intravital microscopy, Mouse models

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Aspergillus fumigatus in vivo imaging

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

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FungiNet total

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Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/home.html

Organisms: Aspergillus fumigatus, Candida albicans

In vivo analysis of temporal and spatial disease progression and immune cell recruitment during invasive Aspergillus fumigatus and Candida albicans infections

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-a3.html

Organisms: Aspergillus fumigatus

FungiNet A - Aspergillus projects

No description specified

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-area-a.html

Organisms: Aspergillus fumigatus

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Julius Maximilians University Würzburg, Department of Internal Medicine II

Country: Germany

City: 97080 Würzburg

Web page: Not specified

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Three-Dimensional Light Sheet Fluorescence Microscopy of Lungs To Dissect Local Host Immune-Aspergillus fumigatus Interactions.

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Aspergillus fumigatus is an opportunistic fungal pathogen that can cause life-threatening invasive lung infections in immunodeficient patients. The cellular and molecular processes of infection during … onset, establishment, and progression of A. fumigatus infections are highly complex and depend on both fungal attributes and the immune status of the host. Therefore, preclinical animal models are of paramount importance to investigate and gain better insight into the infection process. Yet, despite their extensive use, commonly employed murine models of invasive pulmonary aspergillosis are not well understood due to analytical limitations. Here, we present quantitative light sheet fluorescence microscopy (LSFM) to describe fungal growth and the local immune response in whole lungs at cellular resolution within its anatomical context. We analyzed three very common murine models of pulmonary aspergillosis based on immunosuppression with corticosteroids, chemotherapy-induced leukopenia, or myeloablative irradiation. LSFM uncovered distinct architectures of fungal growth and degrees of tissue invasion in each model. Furthermore, LSFM revealed the spatial distribution, interaction, and activation of two key immune cell populations in antifungal defense: alveolar macrophages and polymorphonuclear neutrophils. Interestingly, the patterns of fungal growth correlated with the detected effects of the immunosuppressive regimens on the local immune cell populations. Moreover, LSFM demonstrates that the commonly used intranasal route of spore administration did not result in complete intra-alveolar deposition, as about 80% of fungal growth occurred outside the alveolar space. Hence, characterization by LSFM is more rigorous than by previously used methods employing murine models of invasive pulmonary aspergillosis and pinpoints their strengths and limitations.IMPORTANCE The use of animal models of infection is essential to advance our understanding of the complex host-pathogen interactions that take place during Aspergillus fumigatus lung infections. As in the case of humans, mice need to suffer an immune imbalance in order to become susceptible to invasive pulmonary aspergillosis (IPA), the most serious infection caused by A. fumigatus There are several immunosuppressive regimens that are routinely used to investigate fungal growth and/or immune responses in murine models of invasive pulmonary aspergillosis. However, the precise consequences of the use of each immunosuppressive model for the local immune populations and for fungal growth are not completely understood. Here, to pin down the scenarios involving commonly used IPA models, we employed light sheet fluorescence microscopy (LSFM) to analyze whole lungs at cellular resolution. Our results will be valuable to optimize and refine animal models to maximize their use in future research.

Authors: J. Amich, Z. Mokhtari, M. Strobel, E. Vialetto, D. Sheta, Y. Yu, J. Hartweg, N. Kalleda, K. J. Jarick, C. Brede, A. L. Jordan-Garrote, S. Thusek, K. Schmiedgen, B. Arslan, J. Pinnecker, C. R. Thornton, M. Gunzer, S. Krappmann, H. Einsele, K. G. Heinze, A. Beilhack

Date Published: 4th Feb 2020

Publication Type: Not specified

PubMed ID: 32019790

Citation: mBio. 2020 Feb 4;11(1). pii: mBio.02752-19. doi: 10.1128/mBio.02752-19.

Created: 15th Feb 2021 at 09:39, Last updated: 17th Jan 2024 at 10:24

Dynamic Immune Cell Recruitment After Murine Pulmonary Aspergillus fumigatus Infection under Different Immunosuppressive Regimens

FungiNet A - Aspergillus projects

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Humans are continuously exposed to airborne spores of the saprophytic fungus Aspergillus fumigatus. However, in healthy individuals pulmonary host defense mechanisms efficiently eliminate the fungus. …

Authors: , J. Amich, B. Arslan, S. Poreddy, K. Mattenheimer, Z. Mokhtari, , , ,

Date Published: 13th Jul 2016

Publication Type: Not specified

PubMed ID: 27468286

Citation:

Created: 16th Sep 2016 at 07:46, Last updated: 17th Jan 2024 at 10:24

Surface display of Gaussia princeps luciferase allows sensitive fungal pathogen detection during cutaneous aspergillosis

FungiNet A - Aspergillus projects

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Non-invasive imaging techniques in microbial disease models have delivered valuable insights in the intimate pathogen-host interplay during infection. Here we describe evaluation and validation of a …

Authors: Stefanie Donat, Mike Hasenberg, Tina SchÃ¤fer, Knut Ohlsen, Matthias Gunzer, Hermann Einsele, JÃ¼rgen LÃ¶ffler, Andreas Beilhack, Sven Krappmann

Date Published: 2012

Publication Type: Not specified

PubMed ID: 22286700

Citation:

Created: 27th Jan 2013 at 01:22, Last updated: 17th Jan 2024 at 10:24

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