Long-term adaptation to hypoxic conditions

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.

Differentially synthesized proteins of A. fumigatus under hypoxic vs. normoxic by comparison of protein extracts of the whole mycelium.

Differentially synthesized proteins of A. fumigatus under hypoxic vs. normoxic by comparison of protein extracts of the isolated mitochondria.

Cultivation of A. fumigatus in a oxygen controlled, glucose-limited chemostat under aeration with normal air resulting in a pO2 of 21% oxygen

Cultivation of A. fumigatus in a oxygen controlled, glucose-limited chemostat under aeration with a mixture of nitrogen and normal air resulting in a pO2 of 0.2% oxygen