2D gel images

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.

M Vödisch, K Scherlach, R Winkler, C Hertweck, H-P Braun, M Roth,H Haas, E R Werner, A A Brakhage,O Kniemeyer. Analysis of the Aspergillus fumigatus proteome reveals metabolic changes and the activation of the pseurotin A biosynthesis gene cluster in response to hypoxia. J. Proteome Res., 2011, 10 (5), 2508–2524

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Cultivation of A. fumigatus in a oxygen controlled, glucose-limited chemostat under aeration with a mixture of nitrogen and normal air resulting in a pO2 of 0.2% oxygen