2D gels

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

In project Z2 a sophisticated proteome analysis platform will be provided based on 2D-gel electrophoresis (2D-GE), MALDI-TOF and LC-MS/MS techniques to specific A and Cprojects.

C Baldin, V Valiante, T Krüger, L Schafferer, H Haas, O Kniemeyer, A A Brakhage. Comparative proteomics of a tor inducible Aspergillus fumigatus mutant reveals involvement of the Tor kinase in iron regulation. Proteomics. 2015 Jul;15(13):2230-43. doi: 10.1002/pmic.201400584. Epub 2015 May 26.

Cultivation of A. fumigatus xylp-tor for 16 hours on AMM + 1% (w/v) xylose, for additional 4 hours on AMM without xylose