Austin Mottola

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About Austin Mottola:

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SEEK ID: https://funginet.hki-jena.de/people/139

Location: Germany

ORCID: Not specified

Joined: 28th Jun 2017

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Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

The yeast Candida albicans and the filamentous fungus Aspergillus fumigatus are by far the most important causes of life-threatening invasive mycoses in Europe. Despite the increasing incidence of these infections, the current diagnosis is still difficult and often too late, and options for therapies are limited. Moreover, A. fumigatus and C. albicans have developed multiple sophisticated, specific and unique pathogenicity mechanisms. Many of these mechanisms are not well understood. Research on ...

Projects: FungiNet total, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, INF, A1, A2, A3, A4 (E), A5, B1, B2, B3 (E), B4, C1, C2, C3, C4 (E), C5, C6 (E), Z2, Z1, B5, A6, A7, A8, C7

Web page: http://www.funginet.de/home.html

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FungiNet total

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Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/home.html

Organisms: Aspergillus fumigatus, Candida albicans

Regulation of Candida albicans virulence traits by protein kinases

Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-c2.html

Organisms: Candida albicans

FungiNet C - Candida projects

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Programme: Collaborative Research Center / Transregio 124 "Pathogenic fungi and their human host: Networks of Interaction - FungiNet"

Public web page: http://www.funginet.de/project-area-c.html

Organisms: Candida albicans

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Julius Maximilians University Würzburg, Institute for Molecular Infection Biology

Country: Germany

City: Würzburg

Web page: http://www.imib-wuerzburg.de/imib

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The zinc cluster transcription factor Czf1 regulates cell wall architecture and integrity in Candida albicans.

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The fungal cell wall is essential for the maintenance of cellular integrity and mediates interactions of the cells with the environment. It is a highly flexible organelle whose composition and organization …

Authors: A. Mottola, B. Ramirez-Zavala, K. Hunniger, O. Kurzai, J. Morschhauser

Date Published: 15th Apr 2021

Publication Type: Not specified

PubMed ID: 33860578

Citation: Mol Microbiol. 2021 Apr 15. doi: 10.1111/mmi.14727.

Created: 14th May 2021 at 10:22, Last updated: 17th Jan 2024 at 10:24

Generation of Viable Candida albicans Mutants Lacking the "Essential" Protein Kinase Snf1 by Inducible Gene Deletion.

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The protein kinase Snf1, a member of the highly conserved AMP-activated protein kinase family, is a central regulator of metabolic adaptation. In the pathogenic yeast Candida albicans, Snf1 is considered …

Authors: A. Mottola, S. Schwanfelder, J. Morschhauser

Date Published: 19th Aug 2020

Publication Type: Not specified

PubMed ID: 32817381

Citation: mSphere. 2020 Aug 19;5(4). pii: 5/4/e00805-20. doi: 10.1128/mSphere.00805-20.

Created: 16th Feb 2021 at 13:52, Last updated: 17th Jan 2024 at 10:24

An Intragenic Recombination Event Generates a Snf4-Independent Form of the Essential Protein Kinase Snf1 in Candida albicans.

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The heterotrimeric protein kinase SNF1 plays a key role in the metabolic adaptation of the pathogenic yeast Candida albicans It consists of the essential catalytic alpha-subunit Snf1, the gamma-subunit … Snf4, and one of the two beta-subunits Kis1 and Kis2. Snf4 is required to release the N-terminal catalytic domain of Snf1 from autoinhibition by the C-terminal regulatory domain, and snf4Delta mutants cannot grow on carbon sources other than glucose. In a screen for suppressor mutations that restore growth of a snf4Delta mutant on alternative carbon sources, we isolated a mutant in which six amino acids between the N-terminal kinase domain and the C-terminal regulatory domain of Snf1 were deleted. The deletion was caused by an intragenic recombination event between two 8-bp direct repeats flanking six intervening codons. In contrast to truncated forms of Snf1 that contain only the kinase domain, the Snf4-independent Snf1(Delta311 - 316) was fully functional and could replace wild-type Snf1 for normal growth, because it retained the ability to interact with the Kis1 and Kis2 beta-subunits via its C-terminal domain. Indeed, the Snf4-independent Snf1(Delta311 - 316) still required the beta-subunits of the SNF1 complex to perform its functions and did not rescue the growth defects of kis1Delta mutants. Our results demonstrate that a preprogrammed in-frame deletion event within the SNF1 coding region can generate a mutated form of this essential kinase which abolishes autoinhibition and thereby overcomes growth deficiencies caused by a defect in the gamma-subunit Snf4.IMPORTANCE Genomic alterations, including different types of recombination events, facilitate the generation of genetically altered variants and enable the pathogenic yeast Candida albicans to adapt to stressful conditions encountered in its human host. Here, we show that a specific recombination event between two 8-bp direct repeats within the coding sequence of the SNF1 gene results in the deletion of six amino acids between the N-terminal kinase domain and the C-terminal regulatory domain and relieves this essential kinase from autoinhibition. This preprogrammed deletion allowed C. albicans to overcome growth defects caused by the absence of the regulatory subunit Snf4 and represents a built-in mechanism for the generation of a Snf4-independent Snf1 kinase.

Authors: A. Mottola, J. Morschhauser

Date Published: 19th Jun 2019

Publication Type: Not specified

PubMed ID: 31217306

Citation: mSphere. 2019 Jun 19;4(3). pii: 4/3/e00352-19. doi: 10.1128/mSphere.00352-19.

Created: 16th Feb 2021 at 13:54, Last updated: 17th Jan 2024 at 10:24

The Snf1-activating kinase Sak1 is a key regulator of metabolic adaptation and in vivo fitness of Candida albicans.

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The metabolic flexibility of the opportunistic fungal pathogen Candida albicans is important for colonisation and infection of different host niches. Complex regulatory networks, in which protein kinases …

Authors: B. Ramirez-Zavala, A. Mottola, J. Haubenreisser, S. Schneider, S. Allert, S. Brunke, K. Ohlsen, B. Hube, J. Morschhauser

Date Published: 25th Mar 2017

Publication Type: Not specified

PubMed ID: 28337802

Citation: Mol Microbiol. 2017 Jun;104(6):989-1007. doi: 10.1111/mmi.13674. Epub 2017 Apr 18.

Created: 16th Feb 2021 at 14:03, Last updated: 17th Jan 2024 at 10:24

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